Notes and Precautions: Biological spills outside biological safety cabinets will generate aerosols that can be dispersed in the air throughout the laboratory . These spills can be serious. The laboratory should not be reentered to decontaminate and clean up the spill for at least 30 minutes. During this time the aerosol will be settled by stopping the exhaust air ventilation system. Appropriate protective equipment is particularly important in decontaminating spills involving microorganisms that require BSL 2 containment. This equipment includes lab coat with long sleeves, back-fastening gown or jumpsuit, disposable gloves, disposable shoe covers, and safety goggles and mask or full face shield. Use of this equipment will prevent contact with contaminated surfaces and protect eyes and mucous membranes from exposure to splattered materials.
注意事項: 在操作箱以外的泄漏,可產生懸浮微粒,散播於實驗室中。因此,實驗室須要被封閉最少三十分鐘,關閉通風系統以沉降污染物或懸浮微粒。注意 BSL 的要求,使用適當的保護裝備:包括保護外套,手套,鞋套,安全眼罩及面罩,方可防止眼睛和黏膜受感染。
Spill Involving a Microorganism Requiring BSL 1 Containment
- Wear disposable gloves.
- Soak paper towels in disinfectant and place over spill area.
- Place towels in plastic bag for disposal.
- Clean spill area with fresh towels soaked in disinfectant.
BSL1 微泄漏的處理程序
- 穿上手套
- 用濕滿消毒藥水的紙巾等,覆蓋泄漏的地方數分鐘
- 將紙巾放進印有 Biohazard/生物危害 字樣的紅色膠袋
Spill Involving a Microorganism Requiring BSL 2 Containment
- Alert people in immediate area of spill.
- Put on protective equipment.
- Cover spill with paper towels or other absorbent materials.
- Carefully pour a freshly prepared 1 in 10 dilution of household bleach around the edges of the spill and then into the spill. Avoid splashing.
- Allow a 20-minutes contact period.
- Use paper towels to wipe up the spill, working from the edges into the center.
- Clean spill area with fresh towels soaked in disinfectant.
- Place towels in a plastic bag and decontaminate in an autoclave.
BSL2 微生物泄漏的處理程序
- 通知在場所有人及安全聯絡員
- 穿上保護裝備
- 以紙巾或其他吸收物料蓋著泄漏的地方
- 以家用漂白水浸洗泄漏的地方二十分鐘,並避免污染物飛濺
- 用毛巾等吸收泄漏物,由邊緣向中間做起
- 再以消毒藥水徹底清洗現場
- 所有沾有污染物的物品必須經過消毒,如高溫消毒
Additional Site Instructions:
See Laboratory Safety Manuel, Biosafety BS Section 4 Treatment of Spill in Cabinets and BS Section 7 Clean up of Microbiological Spills.
附加資料:
見實驗室安全手冊 - 生物安全的第 3.4 節,操作箱的消毒處理和第 3.7 節清理微生物的洩漏